Product Info Summary
SKU: | A13355-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-RILPL1 Antibody Picoband™
SKU/Catalog Number
A13355-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RILPL1 Antibody Picoband™ catalog # A09697. Tested in WB, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RILPL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13355-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human RILPL1 recombinant protein (Position: E3-K324). Human RILPL1 shares 93.2% and 93.5% amino acid (aa) sequence identity with mouse and rat RILPL1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A13355-1 is reactive to Rilpl1 in Human, Rat
Applications
A13355-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
47.323kDa
Background of Rilpl1
While the function of the Rab interacting lysosomal protein-like 1 (RILPL1) remains unknown, the homologous protein RILP is a small GTPase that controls transport to endocytic compartments, suggesting RILPL1 may have similar functions. However, overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell, while overexpression of RILPL1 had no visible effect on the location or morphology of lysosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Rat
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RILPL1 using anti-RILPL1 antibody (A13355-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human H9C2(2-1) whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RILPL1 antigen affinity purified polyclonal antibody (Catalog # A13355-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RILPL1 at approximately 50 kDa. The expected band size for RILPL1 is at 47 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of 293T cells using anti-RILPL1 antibody (A13355-1).
Overlay histogram showing 293T cells stained with A13355-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RILPL1 Antibody (A13355-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Rilpl1 (Source: Uniprot.org, NCBI)
Gene Name
Rilpl1
Full Name
RILP-like protein 1
Weight
47.323kDa
Superfamily
RILPL family
Alternative Names
FLJ39378; MGC105128; MGC99793; Rab interacting lysosomal protein-like 1; Rab-interacting lysosomal-like protein 1; RILP-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rilpl1, check out the Rilpl1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rilpl1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RILPL1 Antibody Picoband™ (A13355-1)
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No publications found for A13355-1
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